Isolation of Microorganism from Stool Sample of Diarrhea Patient and Effect of Antibiotics and Herbal Extract

The present investigation was based on study of stool sample collected from 15 children suffering from diarrhea, Cholera, Gastroenteritis. It includes biochemical & microbiological examination of bacteria which is considered to be etiological agent of enteric disease. After biochemical, microbiological morphology study and Gram staining technique and using PIB WIN soft ware mainly five types of pathogens (E.coli, Vibrio cholerae, Enterobacter aerogenes, Klebsiella pneumoniae) were identified and their percentage occurrence in the stool sample were determined. Percentage effectiveness or sensitivity of antibiotics disc (Amikacin 30 mcg/disc, Cefepime 30 mcg/disc, Chloramphenicol 30 mcg/disc, Ciprofloxacin 5 mcg/disc, Gentamycin 10 mcg/disc, Imipenem 10 mcg/disc, Tetracyclin 30 mcg/disc, Tobramycin 10 mcg/disc) & different conc. of herbal extract (10μl,20 μl ,30 μl) of Ocimun sanctum, Aegle marmelos, Mentha spicata) were observed on different isolated pathogens from stool sample, in addition these effects were compared.